Failure to detect SARS-CoV-2 by qRT-PCR in hospitalized patients with COVID-19
DOI:
https://doi.org/10.47464/MetroCiencia/vol29/2/2021/23-31Keywords:
qRT-PCR, Antibodies, SARS-CoV-2, COVID-19, ELISAAbstract
For many symptomatic individuals, a single negative qRT-PCR, (one of the several Nucleic Acid Ampiflication Tests: NAATs) is sufficient to exclude the diagnosis of COVID-19, however, if the initial test is negative but the suspicion of COVID-19 remains high and confirmation of the presence of infection is important for the management or control of the infection, is recommended to repeat the test. A negative result does not exclude the possibility of a SARS-CoV-2 infection and should not be used as the sole criterion for treatment or patient management decisions. The combination of qRT-PCR together with a tomography and the clinical-epidemiological characteristics facilitates the management of patients with SARS-CoV-2. This article aims to analyze patients treated of COVID-19 treated in a general hospital in the south of Quito, at the beginning of the pandemic, which presented a negative qRT-PCR test. Antibody detection was performed. These were positive because blood samples were taken about 2 weeks from the onset of symptoms.
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Wu F, Zhao S, Yu B, Chen YM, Wang W, Song ZG, Hu Y, Tao ZW, Tian JH, Pei YY, Yuan ML, Zhang YL, Dai FH, Liu Y, Wang QM, Zheng JJ, Xu L, Holmes EC, Zhang YZ. A new coronavirus associated with human respiratory disease in China. Nature. 2020 Mar;579(7798):265-269. doi: 10.1038/s41586-020-2008-3. Epub 2020 Feb 3. Erratum in: Nature. 2020 Apr;580(7803):E7. PMID: 32015508; PMCID: PMC7094943
Wang C., Horby P.W., Hayden F.G., Gao G.F. A novel coronavirus outbreak of global health concern. Lancet. 2020;395:470–473. doi: 10.1016/S0140-6736(20)30185-9.
Wu F, Zhao S, Yu B, et al. Complete genome characterisation of a novel coronavirus associated with severe human respiratory disease in Wuhan, China. bioRxiv 2020
Ai T, Yang Z, Hou H, Zhan C, Chen C, Lv W, Tao Q, Sun Z, Xia L. 2020. Correlation of Chest CT and RT-PCR Testing in Coronavirus Disease 2019 (COVID-19) in China: A Report of 1014 Cases. Radiology 200642.
Xie X, Zhong Z, Zhao W, Zheng C, Wang F, Liu J. Chest CT for Typical Coronavirus Disease 2019 (COVID-19) Pneumonia: Relationship to Negative RT-PCR Testing. Radiology. 2020;296(2):E41-E45. doi:10.1148/radiol.2020200343
Corman Victor M, Landt Olfert, Kaiser Marco, Molenkamp Richard, Me er Adam, Chu Daniel KW, Bleicker Tobias, Brünink Sebastian, Schneider Julia, Schmidt Marie Luisa, Mulders Daphne GJC, Haagmans Bart L, van der Veer Bas, van den Brink Sharon, W sman Lisa, Goderski Gabriel, Romette Jean-Louis, Ellis Joanna, Zambon Maria, Peiris Malik, Goossens Herman, Reusken Chantal, Koopmans Marion PG, Drosten Christian. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25(3):pii=2000045. https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045
Corman V, Bleicker T, Brünink S, Drosten C. Charité Protocol and preliminary evaluation as of Jan 13, 2020- Virology, Berlin, Germany Diagnostic detection of Wuhan coronavirus 2019 by real-time RT- PCR. https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assay-v1991527e5122341d99287a1b17c111902.pdf Acceso abril 2020
World Health Organization. Laboratory testing for coronavirus disease (COVID-19) in suspected human cases: interim guidance. WHO/COVID-19/laboratory/2020.5. Geneva: WHO; 2020. Disponible en: https://www.who.int/publications-detail/laboratory-testing-for-2019-novel-coronavirus-in-suspected-human-cases-20200117
Shen, M., Zhou, Y., Ye, J., Abdullah Al-Maskri, A. A., Kang, Y., Zeng, S., & Cai, S. Recent advances and perspectives of nucleic acid detection for coronavirus. Journal of pharmaceutical analysis, 2020; 10(2), 97–101. https://doi.org/10.1016/j.jpha.2020.02.010
Noh JY, Yoon SW, Kim DJ, et al. Simultaneous detection of severe acute respiratory syndrome, Middle East respiratory syndrome, and related bat coronaviruses by real-time reverse transcription PCR [published correction appears in Arch Virol. 2017 Dec 22;:]. Arch Virol. 2017;162(6):1617-1623. doi:10.1007/s00705-017-3281-9
Infectious Diseases Society of America. COVID-19 Prioritization of Diagnostic Testing. https ://www.idsociety.org/globalassets/idsa/public-health/covid-19-prioritization-of-dx-testing.pdf (Accessed on May 29, 2020)
Wang Y, Kang H, Liu X, et al. Combination of RT-qPCR testing and clinical features for diagnosis of COVID-19 facilitates management of SARS-CoV-2 outbreak. J Med Virol. 2020 Feb 25. [Epub ahead of print]. DOI:10.1002/jmv.25721
Lieberman JA, Pepper G, Naccache SN, Huang ML, Jerome KR, Greninger AL. Comparison of Commercially Available and Laboratory-Developed Assays for In Vitro Detection of SARS-CoV-2 in Clinical Laboratories. J Clin Microbiol. 2020;58(8):e00821-20. Published 2020 Jul 23. doi:10.1128/JCM.00821-20
Oliveira BA, Oliveira LC, Sabino EC, Okay TS. SARS-CoV-2 and the COVID-19 disease: a mini review on diagnostic methods. Rev Inst Med Trop Sao Paulo. 2020;62:e44. Published 2020 Jun 29. doi:10.1590/S1678-9946202062044
Liu Y, Yan LM, Wan L, et al. Viral dynamics in mild and severe cases of COVID-19. Lancet Infect Dis. 2020;20(6):656-657. doi:10.1016/S1473-3099(20)30232-2
Xie X, Zhong Z, Zhao W, Zheng C, Wang F, Liu J. Chest CT for typical 2019-nCoV pneumonia: relationship to negative RT-PCR testing. Radiology. 2020 Feb 12; [doi: 10.1148/radiol.2020200343].
Li D, Wang D, Dong J, et al. False-Negative Results of Real-Time Reverse-Transcriptase Polymerase Chain Reaction for Severe Acute Respiratory Syndrome Coronavirus 2: Role of Deep-Learning-Based CT Diagnosis and Insights from Two Cases. Korean J Radiol. 2020;21(4):505-508. doi:10.3348/kjr.2020.0146
Chung M, Bernheim A, Mei X, et al. CT Imaging Features of 2019 Novel Coronavirus (2019-nCoV). Radiology. 2020;295(1):202-207. doi:10.1148/radiol.2020200230
Wang Y, Kang H, Liu X, Tong Z. Combination of RT-qPCR testing and clinical features for diagnosis of COVID-19 facilitates management of SARS-CoV-2 outbreak. J Med Virol. 2020;92(6):538-539. doi:10.1002/jmv.25721
Green DA, Zucker J, Westblade LF, et al. Clinical Performance of SARS-CoV-2 Molecular Tests. J Clin Microbiol. 2020;58(8):e00995-20. Published 2020 Jul 23. doi:10.1128/JCM.00995-20
Wang W, Xu Y, Gao R, et al. Detection of SARS-CoV-2 in Different Types of Clinical Specimens. JAMA. 2020;323(18):1843-1844. doi:10.1001/jama.2020.3786
Yu F, Yan L, Wang N, et al. Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients. Clin Infect Dis 2020; 71:793.
Kucirka LM, Lauer SA, Laeyendecker O, Boon D, Lessler J. Variation in False-Negative Rate of Reverse Transcriptase Polymerase Chain Reaction-Based SARS-CoV-2 Tests by Time Since Exposure. Ann Intern Med. 2020;173(4):262-267. doi:10.7326/M20-1495
Guo L, Ren L, Yang S, et al. Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19). Clin Infect Dis. 2020;71(15):778-785. doi:10.1093/cid/ciaa310
Furukawa NW, Brooks JT, Sobel J. Evidence Supporting Transmission of Severe Acute Respiratory Syndrome Coronavirus 2 While Presymptomatic or Asymptomatic. Emerg Infect Dis. 2020;26(7):e201595. doi:10.3201/eid2607.201595
Zhao R, Li M, Song H, et al. Early detection of SARS-CoV-2 antibodies in COVID-19 patients as a serologic marker of infection. Clin Infect Dis 2020; doi: 10.1093/cid/ciaa523
Zhang G, Nie S, Zhang Z, Zhang Z. Longitudinal Change of Severe Acute Respiratory Syndrome Coronavirus 2 Antibodies in Patients with Coronavirus Disease 2019. J Infect Dis 2020;222(2):183-188. doi:10.1093/infdis/jiaa229
Yang, Wenjing et al. “The role of imaging in 2019 novel coronavirus pneumonia (COVID-19).” European radiology 2020;30(9):4874-4882. doi:10.1007/s00330-020-06827-4
Araujo-Filho, J. D. A. B., Sawamura, M. V. Y., Costa, A. N., Cerri, G. G., & Nomura, C. H. COVID-19 pneumonia: what is the role of
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